Cell Electrophoresis

Cell Electrophoresis

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This book presents a summary of the application and instrumentation of cell electrophoresis. The method of making cell purification and characterization possible according to the cellular negative surface charge density is discussed, and ideas for future developments are explained. The negative electrostatic forces at cell surfaces provide information about cell-cell interaction, blood vessel sealing, cytokine actions, cell transformation, ion transport phenomena, and other biological phenomena. Recalculations of the physical principles of cell electrophoresis reveal possibilities for removing disruptive factors caused by electrical current, heat, and sedimentation. The introduction of computer technology, the performance of simultaneous two-parameter measurements, and the application of cell-friendly but current-inert buffer systems render the method more reliable and efficient.Sample stream is shown as a slit (circular sample streams are commonly used). ... diffusion, sample input configuration, and fraction collection system. ... and high mobility a€” and the effects of Poiseuille flow and electroendosmosis when sample is injected at the bottom, electrophoresis is to the right, and fractions are collected at the top. ... The first problem is the suspension of cells in the inlet pump, the second is the geometry of the Figure 4-14. A thicker L M H free flow electrophoresis.


Title:Cell Electrophoresis
Author: Johann Bauer
Publisher:CRC Press - 1994-09-09
ISBN-13:

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