Circulating Nucleic Acids in Plasma and Serum

Circulating Nucleic Acids in Plasma and Serum

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DNA and RNA fractions have been isolated from the whole blood, serum, plasma, the surface of blood cells, urine, saliva and spinal fluid from both healthy individuals and clinical patients. Recent developments are presented concerning the isolation, quantification and analysis of these molecules and their use in the identification of specific nucleic acid fragments related to a variety of clinical disorders thereby permitting their early diagnosis and prognosis.DNA was extracted from 400 I¼l plasma with the High Pure PCR Template Preparation Kit (Roche Diagnostics, Mannheim, Germany) ... (CRP) levels were determined by an autoanalyzer (Cobas Integra 800, Roche, Mannheim, Germany ) using the manufacturera#39;s kits. ... and plasma fibronectin concentration by nephelometry (Dade Behring, Marburg, Germany), according to the manufacturera#39;s instructions.

Title:Circulating Nucleic Acids in Plasma and Serum
Author: Peter B. Gahan
Publisher:Springer Science & Business Media - 2010-10-08

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