Gene Expression in the Lungs of Ascites-susceptible and -resistant Broilers Undergoing Inflammatory Responses to Entrapped Microparticles

Gene Expression in the Lungs of Ascites-susceptible and -resistant Broilers Undergoing Inflammatory Responses to Entrapped Microparticles

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Intravenous (i.v.) microparticle (MP) injection is a method for selecting broilers with resistance to pulmonary hypertension syndrome (PHS, ascites). In addition to causing pulmonary hypertension, i.v. injected MPs cause a localized inflammation in the lungs of broilers. The objective of this study was to determine the nature of the inflammatory response generated in lungs from MP injected broilers known to be PHS resistant (RES line) or susceptible (SUS line). Specifically, the extent of macrophage infiltration and the expression of chemokines, cytokines, and vasoactive mediators were examined at various times post-MP injection. Included were interleukin-8 (IL-8) and K60; IL-1beta, IL-6, IL-4, and interferon-gamma (IFN-gamma); endothelin-1 (ET-1), endothelin receptors type A and B (ETA and ETB); serotonin transporter (SERT), serotonin receptors: 5-hydroxytryptamine 1A (5-HT1A), 5-HT2A, 5-HT1B, 5-HT2B; cyclooxygenase-2 (COX-2); hypoxia inducible factor-alpha (HIF-alpha); endothelial nitric oxide synthase (eNOS) and inducible NOS (iNOS); Rho kinase (ROCK2); and vascular endothelial growth factor (VEGF). Experiment 1 (Chapter II) showed that macrophage infiltration into the lung increased over time in both the RES and SUS lines. Macrophage infiltration and expression of iNOS was higher in the RES line (POne major problem associated with both of the above discussed methods of RNA quantification is that they do not address the quality of RNA directly. Therefore micro fluidics based technologies such as the Agilent 2100 Bioanalyzer and theanbsp;...


Title:Gene Expression in the Lungs of Ascites-susceptible and -resistant Broilers Undergoing Inflammatory Responses to Entrapped Microparticles
Author: Krishna Raj Hamal
Publisher:ProQuest - 2008
ISBN-13:

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